Table 1.
Data sets used in Bayes' calculations in this study
| Data Set | Rationale | Assignment of Likelihood Values: P(B|A) | Noise Threshold | URL |
|---|---|---|---|---|
| Rat IMCD transcriptome (data set 1) | The ability of a phosphatase to dephosphorylate aquaporin-2 depends on whether or not it is expressed in collecting duct cells. | Complements of minimal Bayes' factors (z* = value/noise) | 0.4 | https://esbl.nhlbi.nih.gov/IMCD-transcriptome/ |
| Rat RNA-Seq (data set 2) | If a phosphatase plays a critical role in regulation of aquaporin-2, we expected it to be expressed in all collecting duct segments. | Complements of minimal Bayes' factors derived from χ2 statistic | https://hpcwebapps.cit.nih.gov/ESBL/Database/NephronRNAseq/All_transcripts.html | |
| Mouse mpkCCD transcriptome (data set 3) | The ability of a phosphatase to dephosphorylate aquaporin-2 depends on whether or not it is expressed in collecting duct cells. | Complements of Minimal Bayes' Factors (z* = value/noise) | 0.4 | https://esbl.nhlbi.nih.gov/mpkCCD-transcriptome/ |
| Mouse mpkCCD proteome (data set 4) | The ability of a phosphatase to dephosphorylate aquaporin-2 depends on whether or not it is expressed in collecting duct cells. | Complements of minimal Bayes' factors (z* = value/noise) | 10th percentile value | https://hpcwebapps.cit.nih.gov/ESBL/Database/mpkCCD_Protein_Abundances/ |
| Dot products from mpkCCD subcellular fraction proteomics (data set 5) | The ability of a phosphatase to dephosphorylate aquaporin-2 depends on whether or not it is found in the same subcellular compartment. | Complements of minimal Bayes' factors (z* = value/noise) | median dot product | https://hpcwebapps.cit.nih.gov/ESBL/Database/mpkFractions/proteomic_fractions_linear.html |
| Apical mpkCCD proteome (data set 6) | The ability of a phosphatase to dephosphorylate aquaporin-2 is greater if it is found in the apical region of the cell. | Complements of minimal Bayes' factors derived from χ2 statistic | http://sbel.mc.ntu.edu.tw/mpkCCDqAMP/qAMP.htm#SamePlace | |
| mpkCCD cytoplasmic fraction proteome (Data set 7) | The ability of a phosphatase to dephosphorylate aquaporin-2 depends on whether or not it is found in the cytoplasm. | If found in cytoplasm fraction, P = 0.95; if found only in nuclear fraction, P = 0.2; if not found, P = 0.4 | https://hpcwebapps.cit.nih.gov/ESBL/Database/mNPD/index.html | |
| Rat IMCD cytoplasmic fraction proteome (data set 8) | The ability of a phosphatase to dephosphorylate aquaporin-2 depends on whether or not it is found in the cytoplasm. | If normalized signal >7, P = 0.95; if signal between 0 and 7, P = 0.75; if signal = 0, P = 0.5 | This paper |