Fig. 6.
Spontaneous inactivation of the large-conductance channel. Channel open probability (Popen) was determined in each of 4 cilia left undisturbed for up to 40 min. The standard external solution was used to bathe the cell and to fill the pipette; the cytoplasmic solution contained 3 µM free Ca2+. The holding potentials and numbers of channels were: +10 mV, 1 channel (black); +30 mV, 1 channel (blue); +80 mV, 1 channel (green); +10 mV, 2 channels (red). Each open probability was determined from an amplitude histogram from a recording of duration 20 s.