Fig. 2.
Western blot analysis of reconstituted fibers. Reconstituted muscle fibers were solubilized in 2.5% SDS solution, as described in materials and methods. Protein samples were loaded and run on an 8% SDS gel to separate various proteins. Proteins were transferred onto a polyvinylidene difluoride membrane and TnT was probed using anti-TnT primary and secondary antibodies. Lanes 1–4: TnTWT + α-MHC, TnTA30V + α-MHC, TnTWT + β-MHC, and TnTA30V + β-MHC, respectively. Lane 5: purified c-myc-tagged recombinant TnT and endogenous (native) TnT proteins.