Fig. 5.
TnTA30V-mediated impact on pCa-tension relationships in α- and β-MHC fibers. A comparison of pCa-tension relationships in α-MHC (A) and β-MHC (B) fibers is shown. The Hill equation was fit to pCa-tension relationships to estimate pCa50 and nH, as described previously (15, 45). The effect of TnTA30V on pCa50 (C) and nH (D) in α- and β-MHC fibers. Data for each group are expressed as means ± SE. Error bars are smaller than symbols in some cases. Statistical differences were analyzed for TnTA30V, relative to TnTWT, using two-way ANOVA and post hoc Fisher’s LSD tests (***P < 0.001; NS, not significant). The number of fibers measured (from 3 hearts) were as follows: TnTWT + α-MHC (n = 12), TnTA30V + α-MHC (n = 14), TnTWT + β-MHC (n = 11), and TnTA30V + β-MHC (n = 12).