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Mean [Ca2+]i and DAF/nitric oxide (NO) recordings after VEGF165 stimulation in UV Endo. Dual imaging of [Ca2+]i and NO was performed in ex vivo intact UV Endo using simultaneous loading of Fura-2 and DAF-2 fluorescent dyes. A: 10 ng/ml VEGF165 was added at time 0 and recorded for 30 min. B: UV Endo are pretreated with 10 nM TPA for 30 min before 10 ng/ml VEGF165 addition at time 0. Values are means + SE (n = 6 vessels).
