Fig. 6.
Effect of VEGF165 pretreatment on ATP-stimulated Ca2+ and NO responses in HUV Endo. HUV Endo were loaded with Fura-2 and DAF-2 to simultaneously record changes in [Ca2+]i and NO. A: a 30-min recording is shown with mean levels of [Ca2+]i and NO for 100 μM ATP treatment. B: the vessels were pretreated with 10 ng/ml VEGF165 for 30 min before subsequent 100 μM ATP addition and recording for 30 min. C: the quantification of the data from A and B for peak and sustained (900 s) [Ca2+]i and total NO (at 1,500 s). Significant reductions after VEGF165 pretreatment occur at sustained [Ca2+]i and total NO. D: the proportion of cells yielding each successive Ca2+ burst (peaks) are plotted. For ATP, n = 6 vessels; for VEGF165 + ATP, n = 6 vessels. Results are shown as means +SE. *Significant difference (P < 0.05).