Table 1.
Molecular weight, pI [75], number of basic residues, maximum charge (Zmax), average charge (Zav), extent of proton-transfer with DPA, and effective density values for each protein. Ions formed from 10 mM aqueous ammonium acetate solution unless otherwise noted
| molecular weight (kDa) | pI | number of basic residues | Zmax | Zav | reaction with DPA (120 s) | Effective Density (g/cm3) | ||
|---|---|---|---|---|---|---|---|---|
| −ΔZmax | −ΔZav | |||||||
| cytochrome c | 12.3 | 9.6 | 24 | 8 | 7.1 | 1.0 ± 0.0 | 1.1 ± 0.1 | 0.50 |
| cytochrome c (water) | 12.3 | 9.6 | 24 | 12 | 8.1 | 3.0 ± 0.0 | 1.8 ± 0.1 | 0.44 |
| myoglobin | 17.6 | 7.4 | 32 | 9 | 8.0 | 0.3 ± 0.5 | 0.6 ± 0.3 | 0.52 |
| carbonic anhydrase | 29.1 | 6.8 | 43 | 12 | 9.8 | 0.7 ± 0.5 | 0.4 ± 0.1 | 0.74 |
| carbonic anhydrase (water) | 29.1 | 6.8 | 43 | 19 | 12.9 | 4.3 ± 2.0 | 2.8 ± 0.1 | 0.69 |
| concanavalin A dimer | 51.7 | 6.0 | 48 | 17 | 15.1 | 1.3 ± 0.5 | 1.5 ± 0.5 | 0.58 |
| holo-transferrin (200 mM ammonium acetate) | 79.7 | 5.4 | 64 | 21 | 19.2 | 1.7 ± 0.5 | 1.4 ± 0.3 | 0.60 |