Fig. 2.

Airway wall inflammation and fibrosis induced by Ad-IL-1β is dependent on IL-17RA/IL-17A. WT (A, C, E) or il17ra^−/− mice (B, D, F) were treated with intratracheal Ad-LacZ (A and B) or Ad-IL-1β (C–F). After 14 days, lungs were harvested and assessed histologically by hematoxylin and eosin (H&E, A–D) or Trichrome staining (E, F). WT mice were intratracheally injected with Ad-LacZ (G, H, M, N) or Ad-IL-1β (I–L, O–R) and treated with isotype control (G, I, K, M, O, Q), anti-IL-17RA (H, J, L) or anti-IL-17A (N, P, R). After 14 days airway inflammation and fibrosis were assessed by H&E (G–J, M–P) or Trichrome staining (K, L, Q, R). Experiments were repeated a minimum of 3 times with at least 3 mice/group. Bar = 100 μm.