Table 1.
Antibody information
| Antibody | Species | Source | Clone | Primary | Secondary |
|---|---|---|---|---|---|
| Anti-Pax7 | Mouse | DSHB | Pax7 | 1:1 | Alexa 594, 488 goat-anti mouse 1:500 |
| Anti-laminin | Rabbit | Abcam | ab11575 | 1:500 | Alexa Fluor 488, 647 goat anti-rabbit, 1:500 |
| Anti-MHCI | Mouse | DSHB | A4.951 Slow isoform | 1:1 | Alexa Fluor 488 goat anti-mouse, 1:500 |
| Anti-CD31 | Rabbit | Abcam | ab28364 | 1:30 | Alexa Fluor 647 goat anti-rabbit, 1:500 |
| Anti-MyoD | Mouse | Dako | 5.8A | 1:50 | goat anti-mouse biotinylated secondary antibody, 1:200; streptavidin-594 fluorochrome, 1:250 |
Detailed information on primary and secondary antibodies and dilutions used for immunofluorescent staining of the frozen muscle cross sections.