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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2016 Jul 7;36(9):1847–1853. doi: 10.1161/ATVBAHA.116.308023

Figure 4. Measurement of 1 cm2 biological surface’s capacity to form pro-coagulant complexes and heparin equivalent estimation with the thrombin assay.

Figure 4

(A) Absorbance readings of residual thrombin generated from: native aorta, heparinase-treated aorta and HUVEC monolayer of 1 cm2 surface area. The results are mean ± SEM of 5 independent assays using five different rats and HUVEC culture (n=5). (B) Heparin equivalent weight was estimated via the slope of curve at 2–6 min (blue box in A) against the standard curve in Figure 2B. (C) Inactivated thrombin was estimated via the absorbance at 11 min (end point in A) against the standard curve in Figure 2C. * p < 0.05.