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. 2016 Nov 16;72(2):137–147. doi: 10.1136/thoraxjnl-2016-208406

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Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/

This is an Open Access article distributed in accordance with the terms of the Creative Commons Attribution (CC BY 4.0) license, which permits others to distribute, remix, adapt and build upon this work, for commercial use, provided the original work is properly cited. See: http://creativecommons.org/licenses/by/4.0/

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Transduction efficiency in mouse lung and nose in the presence of anti-human human parainfluenza virus 1 (hPIV1) antibodies. Mice were treated with human Ig (IVIg) intraperitoneally (IP, 400 μL) or by nasal instillation (IN, 100 μL). Controls did not receive IVIg (n=6/group). Twenty-four hours after passive immunisation, mice were transduced with rSIV.F/HN-hCEF-EGFPLux (1E8 TU/mouse). Control mice remained untreated (UT). Luciferase expression was quantified in lung and nose using bioluminescent imaging 24 hours after virus transduction. (A) Representative images for each cohort of mice, (B) luciferase expression nose and (C) lung. Each symbol represents one animal. The horizontal bar indicates the group median. Two independent experiments were performed (n=6/group/experiment) and a representative figure is shown. EGFP, enhanced green fluorescent protein; GFP, green fluorescent protein.