Presence of anti-PARG1 IgG from sera of various cancer patients detected by Western blot analysis with recombinant His-tagged PARG1 protein.We checked sera of other cancer patients including prostate cancer, bladder cancer, melanoma, endometrial cancer, esophageal cancer, colon cancer, and pancreatic cancer, but high level of anti-PARG1 IgG Ab was specifically observed in RCC patients. The detailed information was described in Materials and Methods. Detection samples were described by red.