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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: J Biomol Screen. 2016 Sep 26;21(10):1112–1124. doi: 10.1177/1087057116670068

Figure 6.

Figure 6

Results of single cell calcium responses in NSCs and neurons with NSCs directly seeded in rhVTN-N-supplemented medium in 384-plates. (A): Serial fluorescence images and kinetic tracers of NSCs recorded in a single cell calcium response assay. (B): Serial fluorescence images and kinetic tracers of neurons recorded in a single cell calcium response assay. (C): Comparison of percentage of responding cells determined from the single call calcium response in NSCs and neurons. A positive responding cell was defined by a cell responded to a stimulus with > 3 × SD increase in mean calcium signal of the control cells. The positive responding neural stem cells were 26% for ATP stimulation and 18% for carbachol stimulation, whereas positive neurons were 97%for ATP and 86% for carbachol. Data are represented as mean ± SEM of at least three biological replicates.