FIGURE 8.

Micelle formation and antifungal protection. All diagrams show mean values + SD from three independent experiments. (A) Determination of the critical micellar concentration (CMC) of TC, TDC and bile by using an eosin Y assay. CMC values were calculated from trend lines based on the logarithm of the sample concentration against absorbance at 542 nm. (B) Effect of increasing ionic strength on the CMC of TC. (C) Effect of increasing salt concentration on TC-mediated protection against caspofungin (CAS). Media contain a fixed concentration of either 10 mg/ml TC, 2 μg/ml CAS or a combination of both. (D) Effect of pH on TC- (10 mg/ml) and TDC- (2.5 mg/ml) mediated protection against 1 μg/ml CAS. Data were normalized against control growth in the absence of CAS. (E) CV absorption in the presence of 5 mg/ml TC at different ionic strength. Inlay photo shows the NaCl-dependent decolourisation of cell pellets. The bar diagram visualizes the relative CV absorption from the culture medium after 30 min of incubation. Statistical analyses were performed by the two-tailed Student’s t-test (^∗p < 0.05, ^∗∗∗p < 0.005, NS = not significant).