Figure 10.

Deletion of Cyld in both the hematopoietic and the parenchymal compartment contributes to protection from experimental cerebral malaria. (A–F) A total of 10 × 10⁶ Bone marrow cells isolated from WT and Cyld^−/− mice, respectively, and 10 × 10⁶ cells were adoptively transferred into sublethally irradiated (one dose of 12 Gy) WT and Cyld^−/− mice as indicated. Eight weeks after bone marrow transfer, chimeras were infected with 1 × 10⁶ Plasmodium berghei ANKA (PbA) parasitized red blood cells. (A) The survival of mice was monitored until day 14 postinfection. (B) The percentage of parasitized erythrocytes in the peripheral blood was enumerated daily from Giemsa-stained thin blood smears. Data represent the mean ± SD (n = 4 each). (C,E) Absolute numbers of total CD45⁺ leukocytes in the blood (C) and brain (E) of PbA-infected mice (day 7). (D,F) Absolute number of interferon-γ- and granzyme B-producing CD8⁺ T cells from blood (D) and brain (F) of PbA-infected mice (day 7) after ex vivo restimulation with GAP-50 peptide. (A–F) *p < 0.05 (two-tailed Student’s t-test). Representative data from one of two experiments are shown.