Figure 5.

Activation of PKC-θ and nuclear factor “kappa light-chain enhancer” of activated B cells is augmented in Cyld^−/− CD8⁺ T cells. (A) The gating strategy used to identify CD45⁺ CD3⁺ CD8⁺ T cells is illustrated. (B,D) Histogram overlays show p-PKC-θ (B) and p-p65 (D) expression in CD3⁺ CD8⁺ T cells of uninfected (day 0) and infected (day 7) WT and Cyld^−/− mice. The intracellular mean fluorescence intensity of PKC-θ (C) and p-p65 (E) is shown for WT and Cyld^−/− mice at the indicated time points. Specific staining for WT mice is shown in light gray and for Cyld^−/− mice in dark gray. Data show the mean (±SD) of six mice from one of two experiments.