Figure 7.

Depletion of CD8⁺ T cells rescues mice from experimental cerebral malaria. C57BL/6 Cyld^−/− and WT (n = 6 each) mice were either treated with anti-CD8 antibody or rat IgG starting 3 days before intraperitoneal (i.p.) infection and i.p. 1 × 10⁶ Plasmodium berghei ANKA (PbA)-infected red blood cells. (A) At day 7 postinfection (p.i.), Evans Blue was injected intravenously in mice. The mice were euthanized 1 h later and perfused with saline, and the isolated brains were photographed. Representative images from two independent experiments are shown. (B) Survival was monitored daily until day 14 p.i. (C) The percentage of parasitized erythrocytes in the peripheral blood was enumerated daily from Giemsa-stained thin blood smears. (A–C) *p < 0.05 (two-tailed Student’s t-test) Representative data from two independent experiments are shown.