Figure 8.

Enhanced CD8⁺ T cell responses but reduced CXCR3 expression by CD8⁺ T cells in the spleen of Cyld^−/− mice. (A) Absolute numbers of SQLLNAKYL pentamer⁺ CD44⁺ CD8⁺ T cells of uninfected mice (day 0) and mice infected with Plasmodium berghei ANKA (PbA)-blood stage parasites (1 × 10⁶ intraperitoneally) were determined by flow cytometry in the brains of WT and Cyld^−/− mice at day 7 postinfection (p.i.) (n = 6 each). (B–E) Absolute (B) and relative (C) numbers of interferon-γ-producing CD8⁺ T cells and absolute (D) and relative (E) numbers granzyme B-producing CD8⁺ T cells from uninfected mice (day 0) and PbA-infected mice at day 7 p.i. after ex vivo restimulation with GAP-50 peptide (n = 6 each), *p < 0.05 (two-tailed Student’s t-test). Data from one of three independent experiments are shown. (F) Histogram overlays show data for CXCR3 expression in CD3⁺ CD8⁺ T cells of uninfected (day 0) and infected (day 7) WT and Cyld^−/− mice. (G) The intracellular mean fluorescence intensity of CXCR3 is shown for WT and Cyld^−/− mice at the indicated time points. (A,C,E) ND = non-detectable. (A,C,E) Data show the mean (±SD) of six mice from one of two experiments.