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. 2017 Feb 1;11:3. doi: 10.3389/fnana.2017.00003

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Copyright © 2017 Nagatomo, Suga, Saitoh, Kogawa, Kobayashi, Yamamoto and Yamada.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Double immunolabeling of SNr cells dissociated from the adult wild-type mouse brain with anti-D1R and anti-GFAP antibodies. (A–D) A typical D1R-positive/GFAP-positive SNr astrocyte (filled arrow). A typical D1R-negative (GFAP-negative) SNr neuron is shown in the same field of view (empty arrow). (A), (B), (C), and (D) represent represent D1R immunofluorescence, GFAP immunofluorescence, differential interference contrast image, and a merged image of these, respectively. Nuclear staining with DAPI (blue) was overlaid. Inset in (D) depicts a magnified view of D1R-positive synaptic bouton-like structures on the SNr neuron. D1R-negative small cells bearing no fine process were excluded from the analysis due to too much digestion and/or mechanical damage.