Figure 5.

Bovine myeloid antimicrobial peptide (BMAP)-27, sBAMP-27, BMAP-28, BMAP-34, and Bac1 promote nucleic acid sensing in primary epithelial cells. (A) Bovine cathelicidins (5 µM) were complexed with 0.1 µg/ml poly(I:C) in medium at room temperature for 10 min before being added to bovine turbinate epithelial cells (BT cells). After 20 h incubation, Mx1 and β-actin were detected by western blot. One experiment out of three representative experiments is shown. (B) Cathelicidin dose-dependent induction of Mx1 in presence of poly(I:C). Increasing concentrations of bovine cathelicidins were mixed with 0.1 µg/ml of poly(I:C) in medium for 10 min and added to BT cells. After 20 h of culture, Mx1 and β-actin were detected by western blot. One representative experiment out of two is shown.