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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Differentiation. 2016 Dec 2;93:66–71. doi: 10.1016/j.diff.2016.11.002

Figure 1. CCND1 protein expression localizes with KI67 and CTNNB1-responsive mRNAs in prostatic bud tips.

Figure 1

(A) 18 dpc male and female UGS sections were immunofluorescently stained to detect CCND1 and the UGS epithelial cell marker CTNNB1. Nuclei were stained with DAPI. Staining patterns in each panel represent 3–4 mice. (B) The CCND1 immunolabeling index was determined as the number of CCND1 immunopositive epithelial cells divided by the total number of epithelial cells within a 200X microscopic field. Results are the mean ± SE of three independent samples per group from at least three litters. Asterisks indicate significant differences (p < 0.05). (C) 18 dpc male UGS sections were immunofluorescently labeled to detect CCND1 and KI67. Co-labeled cells are indicated by arrowheads. (D-F) 18 dpc male UGS sections were stained by ISH to visualize mRNAs (purple) for Ccnd1 and the CTNNB1 target genes Wif1 and Axin2. Sections were counterstained by IHC to visualize UGS epithelium marked by e-cadherin (CDH1). Black arrowheads indicate prostatic bud tips. bl: bladder, sv: seminal vesicle.