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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Differentiation. 2016 Dec 2;93:66–71. doi: 10.1016/j.diff.2016.11.002

Figure 4. Proteasome inhibition drives CCND1 and KI67 protein expression in mouse UGS explants.

Figure 4

14 dpc male mouse UGS explants were grown for 4 days in media containing 5α-dihydrotestosterone (DHT, 10 nM) and vehicle (0.1% DMSO) or DHT and the proteasome inhibitor Z-Leu-Leu-Leu-al (10 mM). 5 μm sections were immunofluorescently labeled to visualize (A) CTNNB1 and CCND1 or (B) CTNNB1 and KI67. Cell nuclei were stained with DAPI. (B, D) CCND1 and KI67 immunolabeling indices were determined as number of prostatic bud epithelial bud cells positive for CCND1 and KI67 divided by the number of visible prostatic bud epithelial bud cells. Results are the mean ± SE of three independent samples per group from three litters. Asterisks indicate significant differences (p < 0.05).