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. Author manuscript; available in PMC: 2017 Jun 12.
Published in final edited form as: Nat Genet. 2016 Dec 12;49(2):296–302. doi: 10.1038/ng.3744

Figure 3. ARID1A loss causes defects in SWI/SNF targeting to chromatin.

Figure 3

a) Live cell morphology of HCT116 ARID1A WT, ARID1A+/−, and Arid1a−/− cells in culture;

b) Proliferation measured by MTT assay, error bars show standard deviation of 3 measurements (technical replicates);

c) Invasion measured by Matrigel-chamber based assay, error bars show standard deviation of 4 measurements (technical replicates);

d) Protein levels of ARID1A, E-Cadherin, and β-actin;

e) Protein levels of Vimentin and β-actin (Note: NIH 3T3 fibroblast cells included for positive control);

f) Fold change (log2) in SMARCA4 and SMARCC1 ChIP-Seq signals at SWI/SNF binding sites in Arid1a−/− cells relative to WT;

g) Immunoprecipitation of SWI/SNF complexes using antibodies targeting ARID1A and ARID1B;

h) Protein levels of ARID1B and β-actin following ARID1B knockdown with 3 independent shRNAs;

i) Proliferation following shRNA-mediated ARID1B knockdown measured by MTT assay; error bars show standard deviation of 3 measurements (technical replicates).