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. Author manuscript; available in PMC: 2017 Jun 12.
Published in final edited form as: Nat Genet. 2016 Dec 12;49(2):289–295. doi: 10.1038/ng.3746

Figure 2. SMARCB1 is essential in maintaining the SWI/SNF complex integrity.

Figure 2

a–b. Immunoprecipitation (IP) of the SWI/SNF complex subunits SMARCC1, SMARCA4, or ARID1A from the nuclear extracts of G401 (a) and BT16 (b) cell lines with or without Doxycycline (Dox)-induced SMARCB1 re-expression. Immunoblotted for subunits SMARCB1, SMARCC1, SMARCA4, ARID1A, ARID1B, SMARCC2, SMARCD1, SMARCE1, ACTL6A, and DPF2. Actin is a loading control.

c. Mass spectrometry showing increased recovered peptides of SWI/SNF complex subunits by IP of SMARCA4 and SMARCC1 in G401 cells after SMARCB1 re-expression (Dox vs. No Dox).

d. Glycerol sedimentation (10–30%) assay of SWI/SNF complex (~2MDa) from SMARCB1-deficient G401 cells without (top half) or with Dox (bottom half) immunoblotted for the indicated SWI/SNF complex subunits. BMI1 is a PRC1 complex subunit serving as a control.

e. Immunoprecipitation (IP) of the SWI/SNF complex by SMARCC1 or SMARCA4 from the nuclear extracts of WT or Smarcb1 deficient mouse embryonic fibroblasts immunoblotted for the indicated SWI/SNF complex subunits.