TABLE 2 .

Sequences of primers used in this study

Primer name	Oligonucleotide sequence
PilF-Fwd	5′ACGCCTTGCAAGATCAACCTGATTCCG3′
PilF-Rev	5′TCGAACGCGCCGTTTTCCAGCTGACGC3′
PilF mid-Rev	5′GGCCGGTTTCTTCATTTGCAGCG3′
PilF Clone-Fwd	5′TCATGCATGCATGACTGTACGCGCCGCGCTGG3′
PilF Clone-rev	5′TCATAAGCTTTCATTTTTCCGCCTGGAATTCCTG3′
MinC Fwd	5′GGATACGGCAACTGCACCACCAGCCAG3′
MinC Rev	5′GACGACGTTGACGAAGTCGTACACCAC3′
MinC mid-Rev	5′CGTGGCGGCGACAGACCTCGAGGA3′
PilN 3′-Fwd	5′GCCAACGTGTTCCAACTG3′
PilO Rev	5′CCACGCTGATCTGGATCG3′
PocA Fwd	5′GAATTCGGGGATATGCCACGTGTGGGA3′
PocA Rev	5′AAGCTTTGCGGCGGAATTTCACGCTTTGC3′
PocA mid-rev	5′GAGGATCTCTCCCAGCGG3′
PocA Clone-Fwd	5′TCATGAATTCGTGTGGGAACTGGTTCAAGCCGG3′
PocA Clone-rev	5′TCATAAGCTTTCACGCTTTGCCTTCCTCGACGTAG3′
PilQ Clone-Fwd	5′AGAATTCCAACAGCAGTCTGTACAA3′
PilQ Clone-rev	5′TCATAAGCTTTCAGCGACCGATTGCGATGGCCTG3′
PilQ 910-Fwd	5′CGACCTGAATCTGGTGGC3′
PilQ FRT-Chk Fwd	5′TTGATCATCAACCTGACCGCGCTGTCG3′
PilQ FRT-Chk rev	5′TCATCGGCTTGATGCTGACGGTCAGG3′
pBAD mcs-Fwd	5′AAGTGTCTATAATCACGGCAGA3′
pBAD mcs-Rev	5′TCACTTCTGAGTTCGGCATGG3′
mCherry Rev	5′TCATGCATGCGCTTCCGCCGCTCTTGTACAGCTCGTCCATGCCGC3′
PilQ 5′ Rev	5′TCATTCTAGAGTCCGCGGCGAGCAATGCCGGCGC3′
PilQ N0 Fwd	5′TCATTCTAGAGGCGAGAAACTGTCGCTGAACTTC3′
FimV Clone Fwd	5′GCGGGTACCATGGTTCGGCTTCG3′
FimV Clone rev	5′GCGTCTAGAGGCCAGGCGCTCCA3′