FIG 1 .

Biofilm elaboration and PIA/PNAG production by Staphylococcus aureus TF2758. (A) Biofilm elaboration. Bacteria were grown in trypticase soy broth (TSB) in the presence (Glc+) or absence (Glc-) of 1% glucose. Biofilm elaboration was measured using the polystyrene microtiter plate assay described in Materials and Methods. The averages and standard errors for each sample are shown. (B) PIA/PNAG production. Extracts from overnight cultures were spotted on a membrane, and PIA/PNAG was detected by rabbit anti-PNAG, as described in Materials and Methods. Non-biofilm-elaborating strain ATCC 49775 was used as a control in the comparative microarray analysis. Figures were compiled from separate images on the same film. (C) Comparative gene expression analysis of TF2758 and ATCC 49775. TF2758 gene expression was represented as a fold change from that of ATCC 49775. Two gene clusters exhibiting marked increases in gene expression are colored (red and blue), and these gene clusters are depicted at right (a and b).