TABLE 2 .
Primers used in the present study
| Purpose and primer | Sequence (5′ to 3′) |
|---|---|
| Plasmid and strain construction | |
| robsm-1 | ACAACGCCCTTAATTGTTGCC |
| robsm-2 | GCAACAATTAAGGGCGTTGTTACCAAAG |
| rob-1 | TACCAAGCTTCCTCTAACAACTGTTTTAC |
| rob-2 | CATCAACTAGTTTGTGCGCTATTTCTTC |
| rob-3 | GCTGTTGCAATCATTATCAACTAGTG |
| rob-4 | AGGTAAAGCTTTAGCGTATTGTAGCG |
| robUp-1 | AACTAAGCTTTGCCATCGTACTACTAG |
| robUp-2 | GAGCAAAGACGCATCACAGCGGTCTGCTAAAATGAAATTC |
| robUp-3 | GAATTTCATTTTAGCAGACCGCTGTGATGCGTCTTTGCTC |
| robUp-4 | CGGCAAGCTTAATGAGGATATCAAGACG |
| robDown-1 | AACTAAGCTTATCACTCAGATCACCTTC |
| robDown-2 | GCGGAATCAGGGAGTGGTTCGTGCGCTATTTCTTCAATTC |
| robDown-3 | GAATTGAAGAAATAGCGCACGAACCACTCCCTGATTCCGC |
| robDown-4 | GTAAACAAAAATAAGCTTGGTCAGCC |
| SAOUHSC_2898-1 | AACTAAGCTTATCACTCAGATCACCTTC |
| SAOUHSC_2898-2 | GGCTTGATTCCTTCAGAAACGTGCGCTATTTCTTCAATTC |
| SAOUHSC_2898-3 | GAATTGAAGAAATAGCGCACGTTTCTGAAGGAATCAAGCC |
| SAOUHSC_2898-4 | GCGAATAAAGCTTCATCCATACG |
| SAOUHSC_2899-1 | GCCGTCTTGGGATCCTCATTAAC |
| SAOUHSC_2899-2 | GGATAATCAGCAGCATAAAGCGGTACACCTTTAGGATCTG |
| SAOUHSC_2899-3 | CAGATCCTAAAGGTGTACCGCTTTATGCTGCTGATTATCC |
| SAOUHSC_2899-4 | CTATGGATCCTTCTTCAGTATC |
| SAOUHSC_2900-1 | TTAGGATCCAAAGGTGCGCTCATTATG |
| SAOUHSC_2900-2 | GAATATAACCTAAGTGACCGCCAGGAATAAAGATGAGCAC |
| SAOUHSC_2900-3 | GTGCTCATCTTTATTCCTGGCGGTCACTTAGGTTATATTC |
| SAOUHSC_2900-4 | CTATTTTGGATCCGTTTACAAC |
| icaR-1 | TGGTGAAGCTTGATCAACGATAGTATC |
| icaR-4 | TAATAAAGCTTGATACCATCGTACTC |
| ica-1 | AATTGGATCCTCATTGAACAAGAAGCC |
| ica-2 | TAATACTAGTTGTCCCCCTTGAGCCCATC |
| ica-3 | GATGAAACTAGTTATGAAAATGCTTATCC |
| ica-4 | AATTGTAACACTAAGGATCCACCCTCC |
| SAOUHSC_2898-comF | AGGTGGATCCTTCGAAATGTGCTTGC |
| SAOUHSC_2898-comR | ACATAAGCTTGATCTACCAAGGC |
| Quantitative PCR | |
| gyrB for | AGGTCTTGGAGAAATGAATG |
| gyrB rev | CAAATGTTTGGTCCGCTT |
| icaR for | CGCCTGAGGAATTTTCTG |
| icaR rev | GGATGCTTTCAAATACCAAC |
| icaA for | AGTTGTCGACGTTGGCTAC |
| icaA rev | CCAAAGACCTCCCAATGT |
| icaD for | ACCCAACGCTAAAATCATCG |
| icaD rev | GCGAAAATGCCCATAGTTTC |
| icaB for | ATACCGGCAACTGGGTTTAT |
| icaB rev | TGCAAATCGTGGGTATGTGT |
| icaC for | CTTGGGTATTTGCACGCATT |
| icaC rev | GCAATATCATGCCGACACCT |
| SAOUHSC_2898 for | ATTGACACCTCGTGACGTTG |
| SAOUHSC_2898 rev | CCACTTGATACGTTGACGAC |
| EMSA and DNase I footprint analysis | |
| ica-p-F | ATTGCGTTATCAATAATCTTATCCTTC |
| ica-p-R (5-biotin) | TTGCAATTCCTTTACCTACCTTTC |
| ica-p-R′ | TTGCAATTCCTTTACCTACCTTTC |
| ica-p-F-s1 | ACAAATATTTCCGTTTAATTATAACAAC |
| ica-p-F-s2 | AATCTATTGCAAATTAAAATACTATC |
| 5bp-deletion-2 | TTGTTGTTATAATTAAACGGTTTGTAATTGCAACTTAATT |
| 5bp-deletion-3 | AATTAAGTTGCAATTACAAACCGTTTAATTATAACAACAA |
| rob-p-F | CGTCTTTGCTCTCTAGTTAAAGAC |
| rob-p-R | CTATTCTCTTTTGCATCTTTTCGC |
| T7 promoter-1 Cy3a | TAATACGACTCACTATAGGG |
| Fp-M13-F | GTTTTCCCAGTCACGAC |
| Fp-M13-R 6-FAMb | CAGGAAACAGCTATGAC |
| pET-28a-Rob-F | AGGTGGATCCATGCGAAAAGATGC |
| pET-28a-Rob-R | TAACAAGCTTTTAGTCATTACGTCCCACC |
| pET-22b-IcaR-F | GGAATTCCATATGCACCACCACCACCACCACTTGAAGGATAAGATTATTGATAACGC |
| pET-22b-IcaR-R | CCCAAGCTTTTATTTCTTCAAAAATATATTTAGTAGCG |
a
Cy3 labeled at the 5′ end.
b
6-FAM labeled at the 5′ end.