FIG 1 .

Phylogeny and habitat of Mucispirillum. (A) Phylogenetic tree of M. schaedleri in relation to other cultured members of the Deferribacteres and abundant gut taxa based on the maximum-likelihood method using the 16S rRNA gene and 500 bootstraps. The source of isolation of the members of the Deferribacteres is indicated by color. GenBank accession numbers are shown in parentheses. Scale bar, 0.05 change per nucleotide position. (B and C) Fluorescence in situ hybridization image of the ceca of ASF⁴ mice colonized with M. schaedleri ASF 457 (Cy3, green), showing localization proximal to the mucosa (B) and its almost complete absence in the lumen (C). All bacteria (targeted by the EUB338I-III mix, Cy5) are blue, and DAPI (4′,6-diamidino-2-phenylindole)-stained cells are shown in gray.