Figure 5. DVGLUT, NHEs and the vATPase all contribute to activity‐induced enhanced cytosolic acid efflux in presynaptic terminals.

A, averaged traces of activity‐induced pH_cyto transients in MN13‐Ib terminals of w¹¹¹⁸ larvae before and after depletion stimulation in 50 μM EIPA (n = 9 larvae). B, paired quantification (from A) of mean J_H+ during stimulation, τ_rec and resting pH_cyto in w¹¹¹⁸ larvae before and after incubation in EIPA. C–H, data collected and represented as in A and B with incubation in 1 μM BafA1 (n = 10), 10 μm EB (n = 8) and vehicle (n = 6) as indicated. See Table 1 for values. Stimulation (black bar) was 40 Hz for 8 s in A, C, E and G. Dashed lines are single exponential curves. Light grey box indicates the overlapping pH_cyto range within which J_H+ during stimulation was calculated. Data in B, D, F and H are plotted as mean ± SEM. ^* P < 0.050, paired Student's t test.