Table 1.
Gene symbol | Primers/probes | Oligonucleotide sequence (5’–3’) |
---|---|---|
β‐actin | Forward | AGATGACCCAGATCATGTTTGAGA |
Reverse | ACCAGAGGCATACAGGGACAA | |
Kiss1R | Forward | GCTGGGAGACTTCATGTGCAA |
Reverse | AGCGGGAACACAGTCACATACC |
Oligonucleotide sequences used to quantify Kiss1R mRNA expression in the perinuclear zone–supraoptic nucleus of non‐pregnant and pregnant rats by qRT‐PCR (Fig. 4).