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. 2016 Dec 27;36(3):319–333. doi: 10.15252/embj.201696038

Figure 2. Composition of oskar mRNPs ex vivo .

Figure 2

  • A–B″
    Colocalization of oskMS2‐mCherry (red, A, B) (A′, B′) with Khc‐EGFP (green, A) (A′) or with EmGFP‐Tm1‐I (green B) (B′) in ex vivo ooplasmic preparations. Scale bars represent 5 μm.
  • C
    Fraction of oskMS2‐MCP‐mCherry mRNPs located non‐randomly within a 200 nm distance of one of the indicated GFP‐tagged protein particles in ex vivo ooplasmic preparations. MCP indicates MCP‐EGFP which, like MCP‐mCherry, can bind to MS2 loops. Staufen (Stau) is a dsRNA binding protein and bona fide partner of oskar mRNA (St Johnston et al, 1991, 1992). All values are significantly different from zero (P < 10−3, one‐sample t‐test).
  • D
    Fraction of oskMS2‐mCherry mRNPs colocalizing (max. 200 nm) non‐randomly with Khc‐EGFP particles in wild‐type and Tm1 eg1/Tm1 eg9 ooplasms in the presence of two (white) or one (grey) copy of endogenous Khc.
Data information: (C, D) P‐values of two‐sample t‐tests are indicated above the relevant bar pairs. Numbers indicate the number of particle clusters (160 mRNPs in each) and the number of preparations (in brackets) analysed. Error bars represent 95% confidence intervals.