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. 2017 Jan 4;9(2):265–279. doi: 10.15252/emmm.201606602

Figure EV3. Cell type‐specific gene expression in CM‐G4‐KO mice.

Figure EV3

  1. Expression of the indicated genes assessed by qPCR in the mice as shown; ****P < 0.0001. Data are expressed as mean ± SEM. One‐way ANOVA with Sidak's multiple comparisons test was used to compare groups. Data are expressed as mean ± SEM. One‐way ANOVA with Sidak?s multiple comparisons test was used to compare groups. The number within bars indicates the number of mice analyzed in that particular group.
  2. In situ hybridization to detect Raldh2, Tcf21, or Tbx18 in the myocardium of the indicated mice 7 days after sham surgery or cryoinfarction to assess epicardial activation. Staining in the embryonic mouse heart served as positive (POS) control. Scale bar: 200 μm. A higher magnification of the epicardial region is shown on the right. Scale bar: 20 μm.
  3. c‐kit immunofluorescence staining of a positive control (embryo liver lobes at embryonic day (E) 12) and 7‐day‐old heart tissues of the indicated mice after injury. Scale bars: 20 μm.
  4. Wt‐1 immunofluorescence staining of 7‐day‐old Con and CM‐G4‐KO mice after cryoinjury. Scale bars: 200 μm.
  5. Myocardial immunofluorescence staining for CD3 as a marker of T cells of the indicated mice 7 days after cryoinjury. An embryonic heart section (from E12) was used as positive control. Scale bars: 20 μm.