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. 2017 Jan 4;9(2):265–279. doi: 10.15252/emmm.201606602

Figure 5. Systemic treatment with recombinant IL‐13 rescued the regenerative defects in CM‐G4‐KO mice.

Figure 5

  1. Depiction of the experimental timescale.
  2. Quantification of left ventricular (LV) scar area of mice 7 days after cryoinjury and treated as indicated. **P = 0.0017 between Con and CM‐G4‐KO after PBS treatment and **P = 0.0078 between CM‐G4‐KO mice with PBS or IL‐13 treatment.
  3. Echocardiographic analysis of left ventricular systolic function in the indicated mice 7 days after cryoinjury; *P = 0.0218.
  4. Quantification of Ki67‐ or pH3‐positive cardiomyocytes in mice treated as described in (A); *P = 0.0106 and **P = 0.0052 between Con and CM‐G4‐KO after PBS treatment and *P = 0.0439 or **P = 0.0023 between CM‐G4‐KO mice with PBS or IL‐13 treatment.
  5. Quantification of capillary density as capillary/cardiomyocyte (CM) ratio in mice treated as described in (A). *P = 0.0365, **P = 0.0031.
  6. Quantification of αSMA‐positive small conductance vessel (˜20–50 μm in diameter) in myocardial sections of the mice treated as indicated.
  7. Representative immunoblots for STAT6 and GAPDH from neonatal mouse hearts (KO = CM‐G4‐KO) treated as indicated. The quantification of the blots is shown on the right. *P = 0.0114.
Data information: (B–G) The number within bars indicates the number of mice analyzed in that particular group. All data are expressed as mean ± SEM. One‐way ANOVA with Sidak's multiple comparisons test (B–G) were used to compare groups.Source data are available online for this figure.