Figure EV1. Huwe1 is an intestinal tumour suppressor gene commonly mutated in human CRC.

1. cBioportal OncoPrints of HUWE1 mutation rate in CRC identified during sequencing projects.
2. cBioportal OncoPrints of HUWE1 mutation rates in a range of human cancers.
3. UbcH7 pulldown of HUWE1 HECT domain containing point mutations identified in colorectal cancer sequencing projects.
4. Quantification of total tumour numbers per colon in sacrificed Vil Apc, Vil Apc Huwe1 ^het and Vil Apc Huwe1 ^hom mice. Deletion of Huwe1 led to a significant increase in the number of tumours per colon (Mann–Whitney, n ≥ 3). Mean and standard deviation are plotted.