Figure 2. Huwe1 deletion leads to perturbed intestinal homoeostasis.

1. H&E staining of control and Huwe1‐deleted intestinal epithelium. Shortened villi in Huwe1‐deficient tissue are indicated.
2. BrdU IHC of control and Huwe1‐deleted intestinal epithelium.
3. PAS staining identifying goblet cells. No gross changes were observed.
4. Lysozyme staining (Paneth cell marker) of control and Huwe1‐deleted small intestine. Note the occurrence of lysozyme‐positive cells away from the crypt base (black arrows).
5. Dual periodic acid–Schiff/alcian blue staining to identify Paneth cell secretory vesicles (light blue/pink, marked with black arrows) and goblet cells (dark blue/purple, marked with red arrows). Note Paneth cell secretory vesicles are restricted to crypt base in both control and Huwe1‐deficient small intestines (inset, black arrows).
6. MMP7 staining of control and Huwe1‐deleted small intestine. Note MMP7 staining is restricted to crypt base in Huwe1‐deficient intestines.

Data information: Scale bars = 100 μm.