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. 2016 Dec 28;9(2):198–218. doi: 10.15252/emmm.201606743

Figure 6. ROCK activation promotes collagen degradation.

Figure 6

  1. Representative fluorescence images of cells treated with 1 μM 4HT in the absence or presence of 10 μM H1152 for 18 h on Collagen1‐FITC (Col1‐FITC). Co‐staining for F‐actin, MMP10 (red), and DAPI (blue). Scale bar = 10 μm.
  2. Quantification of collagen degradation by cells treated with 1 μM 4HT or 4HT + 10 μM H1152 for 16 h. Means ± SEM (n = 15), one‐way ANOVA with multiplicity adjusted exact P‐value by post hoc Tukey multiple comparison test.
  3. Quantification of collagen degradation by cells treated with 1 μM 4HT, 4HT + DMSO vehicle, 4HT + 50 μM Blebbistatin or 4HT + 10 μM GM6001 for 16 h. Means ± SEM (n = 15), one‐way ANOVA with multiplicity adjusted exact P‐value by post hoc Tukey multiple comparison test.
  4. Immunofluorescence of collagen matrix sections co‐stained for MMP10 and DAPI. Scale bar = 20 μm.
  5. Mmp13 in situ hybridization‐stained sections of collagen matrices. Scale bar = 50 μm.