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. 2016 Dec 8;36(3):361–373. doi: 10.15252/embj.201592426

Figure 2. RAD52 is essential in the presence of high levels of genomic ribonucleotides present in nascent leading strand DNA in a Top1‐dependent manner.

Figure 2

  1. Tetrad analysis of RAD52/rad52Δ diploids. 1 to 12 are tetrad dissections, and A–D are haploid spore colonies. Plates were imaged after 5 days of growth on YPDA at 30°C.
  2. Microscopy images reveal that the pol2‐M644G rnh201Δ rad52Δ haploid derivatives sporulated but failed to divide more than a few times and arrested as large G2/M cells.
  3. Tetrad analysis of RAD52/rad52Δ diploids in a rnh201‐RED mutant reveals that the presence of unrepaired single ribonucleotides is the cause of synthetic lethality in a pol2‐M644G rnh201Δ or pol2‐M644G rnh201‐RED strain background. Plates were imaged after 4 days of growth. pol2‐M644G rnh201‐RED rad52Δ haploid derivatives arrested as large G2/M cells.