(A) Overall
CAN1 mutation rates for strains with deletions of indicated genes. Spontaneous mutation rates were determined using fluctuation analysis as described previously (Shcherbakova & Kunkel,
1999). (B) Same as in panel (A), but for 5‐FOA‐resistance (5‐FOA
r) conferred by mutation of the
URA3 reporter gene in orientation 2 adjacent to the
ARS306 replication origin (Nick McElhinny
et al,
2008). Displayed are mean values ± the 95% confidence interval. The experiment was performed twice and mutation rate measurements were calculated for 24 independent isolates per genotype per experiment. Displayed are median values ± the 95% confidence interval.