Figure EV2. Oct6 forms a long‐lived homodimer on MORE DNA.

• A–D
  Off‐rate EMSA using the mouse Oct6 POU (A, C) and the mouse Oct4 POU (B, D) along with MORE (A, B) or SoxOct (C, D) DNA elements, respectively. Dissociation was initiated after incubation of proteins with Cy5‐labeled DNA for 1 h by the addition of a 200‐fold excess of unlabeled competitor DNA of the same sequences as the Cy5‐labeled reporter DNA. Reactions were prepared in single tubes and directly loaded onto running gels after indicated time points. Concentrations of the reactants and sequences of the forward DNA strand are mentioned.