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. 2016 Dec 30;18(2):241–263. doi: 10.15252/embr.201642386

Figure EV4. Nup358 depletion does not affect repression of mRNAs tethered with AGO2.

Figure EV4

HEK293T cells were sequentially transfected with control (siControl) or Nup358 siRNA (siNup358), followed by co‐transfection of pRL‐5BoxB construct (a representative schematic is shown on top) with FL as internal control, and HA‐AGO2, N‐HA‐LacZ, or N‐HA‐AGO2, as indicated. Left panel: Graph represents RL/FL luminescence normalized to HA‐AGO2 transfected samples for control and Nup358 knockdown, separately. RL/FL luminescence ratio was calculated. Data are presented as mean ± SD (n = 3). Western blot (right panel) shows the extent of Nup358 knockdown in cells transfected with indicated siRNA and expression constructs, using specific antibodies. Vinculin was used as loading control.