RNase‐free ddH2O (control group) or in vitro‐transcribed mRNAs of myc‐Cd74, myc‐Tab1, myc‐Tnip1, myc‐Psat1, and myc‐Ralgps1 were microinjected into zygotes, and then, the expression of exogenous protein was detected by Western blotting using anti‐myc tag antibody.