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. 2016 Dec 14;18(2):264–279. doi: 10.15252/embr.201643353

Figure 2. EM reconstruction of endogenous Elp123 sub‐complex.

Figure 2

  1. SDS–PAGE gel showing the purified Elp123 sub‐complex used as input for gel filtration. Protein bands marked with asterisks presumably correspond to different phosphorylation states of Elp1.
  2. Representative negative‐stain EM field of the Elp123 sub‐complex. Particles in side and top views are highlighted. Scale bar, 50 nm.
  3. Reference‐free class averages and back‐projections of the Elp123 model. Scale bar, 20 nm.
  4. EM reconstruction of the Elp123 sub‐complex at 27 Å resolution.
  5. Fitting of the Elp1 CTD into the Elp123 reconstruction.
  6. Fitting of the Elp2 crystal structure in the difference density generated by subtracting the partial Elp123 reconstruction from the complete Elp123 reconstruction.