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. 2016 Dec 14;18(2):264–279. doi: 10.15252/embr.201643353

Figure 5. Validation of the Elp123 and holoElongator models.

Figure 5

  1. GST pull‐down assays of purified GST‐tagged Elp3 SAM domain (aa72–389) and KAT domain (aa348–557) with untagged full‐length Elp1 and Elp451–2706. GSH resin and proteins were used as input controls. Lower panel shows 5% of the input and upper panel shows bound fractions. All samples were analyzed by SDS–PAGE and stained by Coomassie. Identities of respective proteins are indicated on the right.
  2. Same as (A), but using only GST‐tagged Elp3 SAM domain with combinations and variants of Elp1, Elp2, and Elp456.
  3. Same as (A) using GST‐tagged full‐length Elp1 and truncations and deletions of Elp456.
  4. Same as (A) using GST‐tagged C‐terminally truncated Elp1 in combination with truncated versions of Elp4.