Figure 2. AFM images of aggregates of insulin and BSA obtained from eugenol-inhibited aggregation reactions.

(a) AFM images of insulin mature fibrils obtained from an inhibited aggregation reaction which reveal a diameter value of ~95 nm (see inset). (b) Mature amyloid fibrils of BSA obtained from an inhibited aggregation reaction which show a diameter value of ~120 nm (see inset). (c) Occurrence of spheroidal oligomers of insulin with an average diameter of ~60 nm (see inset). (d) Morphology of oligomers or protofibrils of BSA shows an average diameter of ~100 nm. Mature aggregates of proteins in the absence of eugenol are shown in Supplementary Figures S2, and S3.