Table 3. Serum proteins differentially expressed 5 weeks post-surgery by 2-DE analysis in the group of CCI-saline and identified by Q-ToF LC/MS.
| Entry name(a) | Protein full name | Acc. number(b) | Gene name | MW (Da) | Score(c) | Peptide match/sig. match(d) | Seq/ sig. seq(e) | Seq. cov(f). | emPAI(g) | Fold change(h) | Fold change(i) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| VDBP | Vitamin D-binding protein | P04276 | Gc | 55106 | 642 | 52/37 | 14/10 | 30% | 1.01 | +8.5 | 0.96 |
| A1M | Alpha-1-macroglobulin | Q63041 | A1m | 168388 | 4989 | 299/218 | 17/15 | 13% | 0.62 | +6.1 | 0.84 |
| APOA1 | Apolipoprotein A-I | P04639 | Apoa1 | 30100 | 1083 | 90/69 | 23/20 | 59% | 14.29 | +22.6 | +1.92 |
| PTGDS | Prostaglandin-D H2-isomerase | P22057 | Ptgds | 21301 | 709 | 42/36 | 6/5 | 31% | 3.71 | +7.6 | +1.45 |
| TTR | Transthyretin | P02767 | Ttr | 15824 | 750 | 45/32 | 11/8 | 59% | 7.42 | +20.4(j) | no detect(k). |
| APOE | Apolipoprotein E | P02650 | Apoe | 35788 | 4187 | 230/182 | 27/20 | 64% | 14.63 | +3.3 | +2.8 |
(a)Protein entry name (all with extension_RAT) from UniProtKB database, conforming to Fig. 4.
(b)Primary accession number from UniProtKB database.
(c)The highest scores by MASCOT search engine.
(d)Total number of matched peptides and the significant matches.
(e)Total number of sequences and the number of significant sequences.
(f)Percentage of amino acids sequenced for the detected protein.
(g)Exponentially modified protein abundance index.
(h)Protein spot intensity change in CCI-saline vs sham-operated saline.
(i)Protein spot intensity change in CCI-indomethacin vs sham-operated saline.
(j)Fold change was calculated towards CCI-indomethacin since sham-operated rats had no detectable spot.
(k)Fold change was not calculated since sham-operated rats had no detectable spot.