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. 2017 Feb 1;7:41358. doi: 10.1038/srep41358

Figure 2. Bone marrow cells from mice treated with Clomipramine have increased osteoclast forming potential ex vivo.

Figure 2

WT mice were treated with Clomipramine, as in Fig. 1. Bone marrow cells were used. (a) Cells were stained with anti-CD11b and Gr1- Abs for the percentage of CD11b+Gr1−/low OC precursors or anti-CD45 and CD105 Abs for the percentage of CD45-CD105+ mesenchymal progenitors. Values are mean ± S.D. of 5 to 7 mice/group. (b) Cells were cultured with M-CSF and RANKL for 7 d in OC formation assays and stained for TRAP activity. Photos show TRAP+ OCs. TRAP+ OC number was assessed. Values are mean ± S.D. of 4 wells/treatment. Experiments = 3. (c) Cells were cultured in osteoblast-inducing medium for 21 d. The number of CFU-ALP+ colonies was counted. Values are mean ± S.D. of 4 wells/treatment. Experiments = 3. *p < 0.05 versus Ctrl. Ctrl: saline, CLP: Clomipramine.