Table 3. Physiological parameters obtained from aerobic batch fermentation in mineral medium supplemented with amino acids.
| Strain | Physiological parameters |
|||||
|---|---|---|---|---|---|---|
| μmax (h−1) | Viability (%) | Final biomass1 (g dw/L) | YBiomass/substrate (cmol × cmol−1) | YEtOH/substrate (cmol × cmol−1) | YGlycerol/substrate (cmol × cmol−1) | |
| wt | 0.44 ± 0.00 | 100 ± 0 | 5.0 ± 0.1 | 0.12 ± 0.00 | 0.43 ± 0.01 | 0.013 ± 0.000 |
| ELO3, AUR1-OE | 0.44 ± 0.01 | 100 ± 0 | 5.1 ± 0.1 | 0.12 ± 0.00 | 0.44 ± 0.04 | 0.012 ± 0.000 |
| orm1/2Δ | 0.37 ± 0.03* | 89 ± 1* | 3.9 ± 0.1* | 0.13 ± 0.01 | 0.33 ± 0.04* | 0.019 ± 0.000* |
| ELO3, AUR1-OE, orm1/2Δ | 0.42 ± 0.00# | 97 ± 2 | 4.6 ± 0.0*# | 0.14 ± 0.01* | 0.40 ± 0.01# | 0.014 ± 0.000# |
1Final biomass concentration when all carbon sources had been depleted, measured in gram dry weight per litre culture. Results were calculated from three biological replicates and are given as the mean ± standard deviation. Differences obtained using one-way ANOVA with post-hoc Tukey HSD for multiple pairwise comparisons (p < 0.05). *Significant difference compared to wild type, #Significant difference compared to orm1/2Δ.