Skip to main content
. 2017 Feb 1;7:41376. doi: 10.1038/srep41376

Figure 1.

Figure 1

Effect of retinoid derivatives on the transport activity of Pgp (a) and ABCG2 (b) expressed in NIH 3T3 and MDCK cells, respectively. Cells were pre-treated with retinoids or transporter inhibitors (cyclosporin A or Ko143) for 20 min at 37 °C and then the NIH 3T3/NIH 3T3 MDR1 cell line pair was stained with 500 nM calcein-AM for 20 min (a), while the MDCK/MDCK ABCG2 cell pair (b) was stained with 5 μM mitoxantrone for 40 min at 37 °C. The calcein or mitoxantrone accumulation of the transporter expressing cells is shown as a percentage of that of the transporter negative cells. Mean ± SD values of three independent experiments are shown. The expression levels of the transporters were determined by direct immunofluorescence using Alexa 647 conjugated 15D3 mAb for Pgp and 5D3 mAb for ABCG2 (c). (*significant difference compared to the control, P < 0.05 by ANOVA, Holm-Sidac post-hoc analysis).