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. 2017 Feb 1;7:41892. doi: 10.1038/srep41892

Figure 6. Impact of ATM and ATR kinase inhibition on survival and intestinal crypt health in Cdkn1a(p21CIP/WAF1)−/− mice.

Figure 6

(A) Kaplan-Meier survival curves for Cdkn1a(p21CIP/WAF1)−/− mice treated with vehicle or 100 mg/kg AZ31 2 h prior to 9 Gy TBI. N = 5 mice per cohort. (B,C) Cdkn1a(p21CIP/WAF1)−/− mice received vehicle, 100 mg/kg AZ31, or 75 mg/kg AZD6738 2 h prior to 9 Gy TBI. Small intestine tissues were harvested at 7 days after TBI. The total number of crypts per circumference of small intestine (B) and the number of regenerated crypts per circumference of small intestine (C) were enumerated. Box and whisker plots depict 12–16 total circumferences (n = 12–16), with 6–8 circumferences from each of 2 mice. ****p < 0.0001. (D) Cdkn1a(p21CIP/WAF1)−/− mice received vehicle, 100 mg/kg AZ31, or 75 mg/kg AZD6738 2 h prior to 9 Gy TBI. Small intestine tissues were harvested at 4 h after TBI. For un-irradiated mice, tissues were harvested at 6 h after inhibitor dosing, equivalent to 4 h after TBI. The number of TUNEL positive cells per small intestine crypt was enumerated. Box and whisker plots depict counts from a total of 200 crypts (n = 200), with 100 crypts from each of 2 mice. ***p < 0.001, ****p < 0.0001.