Figure 6. N-BODIPY fluorescence correlates with the number of peroxisomes in cells.

(A) Time-course of N-BODIPY fluorescence in total extracts from BY-2 cells treated with 1 mM clofibrate. (B) N-BODIPY staining of cells treated with 1 mM clofibrate for 30 min. Scale bar 10 μm. (C) Density of peroxisomes in control and cells treated with clofibrate for 30 min (number of cells analyzed N=25). (D) Frequency of peroxisomal sizes. White bars, control; black bars, clofibrate (number of peroxisomes measured N = 150). (E) Time-course of N-BODIPY fluorescence in total extracts from BY-2 cells treated with 30 mM H₂O₂. (F) Staining of peroxisomes in cells treated with H₂O₂ for 30 min. Scale bar, 10 μm. (G) Density of peroxisomes in control and cells treated with H₂O₂ for 30 min (number of cells analyzed N = 29). (H) Frequency of peroxisomal sizes. White bars, control; black bars, H₂O₂ (number of peroxisomes measured N = 400). (I) N-BODIPY fluorescence in the total extracts from leaves of Col-0 and atg5-1 plants (number of plants measured N = 5). Error bars show standard deviation. The difference between mean values denoted by the same letter is insignificant (P > 0.05, one-way ANOVA test in A and B, or t-test in C, G, and I).